Physiochemical Changes to TTCF Ensilication Investigated Using Time-Resolved SAXS

Successful eradication or control of prevailing infectious diseases is linked to vaccine efficacy, stability, and distribution. The majority protein-based vaccines are transported at fridge (2–8 °C) temperatures, cold chain, retain potency. However, this has been shown be problematic. Proteins inherently susceptible thermal fluctuations, occurring during transportation, causing them denature. This leads ineffective an increase in vaccine-preventable diseases, especially low-income countries. Our research utilises silica preserve room temperature, removing the need for chain logistics. methodology based upon sol–gel chemistry which soluble employed encapsulate ensilicate proteins. yields a protein-loaded nanoparticle powder stored temperature subsequently released using fast chemical process. We have previously that tetanus toxin C fragment (TTCF) ensilication diffusion-limited cluster aggregation (DLCA)-based process time-resolved small-angle x-ray scattering (SAXS). Here, we present our expanded investigation on modularity system further understanding via SAXS. results show variations could prove useful transition from batch in-flow manufacturing ensilicated nanoparticles.